期刊文献+

硅钼杂多酸单扫示波极谱法测定半胱氨酸 被引量:1

Single Sweep Oscilloscopic polarography Determination of Cysteine Based on its Reaction with 12-Molybdosilicate
下载PDF
导出
摘要 研究了硅钼杂多酸与半胱氨酸的反应,其产物在氨—氯化铵缓冲介质中,于滴汞电极上,在Epa≈-0.96V(VS.SCE)处,产生灵敏吸附波。其阳极化导数波高与半胱氨酸浓度在1×10-7~5×10-5mol/L呈线性关系,检测限为5×10-8mol/L。本法对混合样品中半胱氨酸的测定,回收率在98.8%~101.2%之间变化,相对标准偏差为0.6%~1.13%。 A new method for the determination of trace amounts of cysteine has been reported. The method is base on measurement of the substace produced by the reaction of cysteine of the substace produced by the reaction of cysteine with 12-molybdosilicate,which can be strongle absorbed on DME and produced a anodic wave at -9.6v(Vs.SCE) in NH 3-NH 4Cl buffer solution (pH=9.6). The derivative peak height is proportional to the concentration of cysteine in the range of 1×10 -7  ̄5×10 -5 mol/L.The detection limit of 5×10 -8 mol/L and a recovery of 98.8% ̄101.2% and the relative standard deviation of 0.6% ̄1.13% can be a chieved when this mothod is used to determine cystieine in mixed sample.
出处 《信阳师范学院学报(自然科学版)》 CAS 1998年第1期51-53,共3页 Journal of Xinyang Normal University(Natural Science Edition)
基金 河南省教委资助
关键词 硅钼杂多酸 示波极谱法 半胱氨酸 测定 Silicon-molybdenum heteropoly acid Oscilloscopic polarography Cysteine
  • 相关文献

参考文献2

共引文献4

同被引文献3

  • 1曾飒,谭辉玲.L-半胱氨酸的停流流动注射动力学快速测定法[J].分析测试学报,1996,15(3):78-81. 被引量:5
  • 2Chwatko G,Bald E.Determination of Cysteine in Human Plasma by High-Performance Liquid Chromatography[J].Talanta,2000,52(3):509-515.
  • 3Dimas A M,Kelly C L,Cassia T B V.Spectrophotometic Determination of Cysteine and/or Carbocysteine in a Mixture of Amino Acid,Shampoo,and Pharmaceutical Product Using p-Benzoquinone[J].Talanta,1999,50(5):1003-1010.

引证文献1

二级引证文献2

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部