摘要
目的观察应用壳聚糖治疗大鼠胸髓完全横断损伤后,损伤区及其周围小胶质细胞/巨噬细胞的变化情况。方法35只成年雌性Wistar大鼠随机分成假手术组(n=5)、单纯损伤组(n=15)、壳聚糖治疗组(n=15)。分别于术后1d、3d、7d、2周和4周通过免疫组织化学方法,检测小胶质细胞/巨噬细胞的变化情况。结果单纯损伤后小胶质细胞/巨噬细胞在1d时即活化增殖,3d时达高峰,之后迅速减少;应用壳聚糖治疗后,7d达高峰,2～4周时仍有OX42阳性小胶质细胞/巨噬细胞存在。结论壳聚糖可更长时间地募集小胶质细胞/巨噬细胞到损伤区及其周围脊髓组织。
Objective To observe the response of activated microglia/macrophages within and around the lesion site after rats＇ spinal cord injury treated with chitosan. Methods 35 adult female Wistar rats were randomly divided into the sham group （n=5）, completely transection group （n= 15） and chitosan treated group （n= 15）. The responses of activated microglia/macrophages on the 1st day, 3rd day, 7th day, and in the 2nd week and 4th week after surgery were examined by immunohistochemistry. Results Microglia/ macrophages were fully activated and the number of these cells increased and got the highest level on the 3rd day post injury, then quickly decreased. In the chitosan treated animals, the microglia/macrophages were fully activated and the number increased to peak on the 7th day, then quickly decreased, there were still a few microglia/macrophages around the wound in the 2nd to 4th week after the treatment. Conclusion Microglia/macropbages could be recruited within and around the lesion area longer when treated by chitosan.
出处
《中国康复理论与实践》
CSCD
2009年第4期321-323,共3页
Chinese Journal of Rehabilitation Theory and Practice
基金
"十一五"863计划生物和医药技术领域干细胞与组织工程重大项目(No.2006AA02A129)
