滇黄芩器官发生与不同继代次数遗传稳定性的RAPD分析

Organogenesis of Scutellaria amoena and Genetic Stability of Regenerated Plantlet Analyzed by RAPD

本研究以滇黄芩不带腋芽茎段为外植体,采用不同配比激素的MS培养基建立了滇黄芩器官发生,植株再生体系。结果表明,茎段在MS+6-BA 2mg/L+NAA 0.5mg/L培养基上培养时,愈伤组织诱导率达到100%;以相同培养基进行分化,并在MS+6-BA 2mg/L+NAA 0.2mg/L培养基上扩繁丛生芽。在1/2MS+IBA 0.2mg/L培养基诱导生根,扦插继代。利用RAPD分析不同继代次数再生植株,所用的39条随机引物中只有1条引物带型发生变化。说明经组织培养获得的再生植株遗传稳定,可多次继代培养,为滇黄芩进一步遗传操作和扩大药材资源奠定基础。

Organogenesis and plant regeneration system was established ofScutellaria omoena C.H.Wright on MS medium with different phytohormones. Non-axillary bud stems fregrnents were used as the explants. MS medium added with 2 mg/L 6-BA and 0.5 mg/L NAA was optimal for callus induction. The frequency of callus induction reached to 100%. Buds were differentiated on the same medium, and grew up on MS medium with 2 mg/L 6-BA, 0.2 mg/L NAA and 20 g/L sucrose. Regenerated shoots were rooted and subcultured on half strength of MS medium supplemented with 0.2 mg/L IBA. The genetic stability of regenerated plants was analysed using RAPD markers. The results of RAPD amplification showed that the genetic stability of the regenerated plantlets with repeatedly subculturing was maintained, though slight variations were found. This suggested that in vitro organogenesis were a good rapid propagation way to obtain genetic stable descends ofScuteUaria omoena C.H.Wright.