OJ-H柱拆分西酞普兰对映异构体

Direct resolution of citalopram enantiomers by chiral stationary phase using CHIRAEL OJ-H column

目的:利用CHIRALCEL OJ-H手性柱,建立了直接拆分西酞普兰对映异构体的方法。方法:考察了流动相中不同醇类改性剂、柱温、有机碱添加剂对西酞普兰对映异构体拆分的影响。优化后的色谱条件为:CHIRALCEL OJ-H手性柱(250 mm×4.6 mm,5μm);以正己烷-无水乙醇-二乙胺(80∶20∶0.1)为流动相;流速为0.5 mL.min-1;检测波长为240 nm;柱温为室温。结果:R-和S-西酞普兰的保留时间分别为12.9和14.3 min,能够达到基线分离(R>1.5)。结论:此方法方便、准确,可用于西酞普兰的药品质量控制。

Objective：To develop a direct resolution method for citalopram enantiomers using a CHIRALCEL OJ-H column（250 mm ×4.6 mm,5μm）. Methods：The mobile phase consisted of n-hexane-alcohol-diehyl- amine（80： 20：0.1 ） at a flow rate of 0.5 mL.min^-1. The detection wavelength was 240 nm. The enantiomers were separated at room temperature. The influence of different kind of alcohol, column temperature and the kind of organic base additives were evaluated. Results：The retention times of R-citalopram and S-citalopram were 12.9 rain and 14.3 min,respectively. Baseline separation was obtained （ R 〉 1.5 ）. Conclusion： This method is simple, accurate and suitable for the quality control of citalopram.