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采用扩增核糖体DNA限制性分析(ARDRA)研究双孢蘑菇培养料后发酵过程中的细菌群落结构(Ⅱ)-细菌群落结构ARDRA分析 被引量：2

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摘要 采用ARDRA对双孢蘑菇[Agaricus bisporus(Large)Sing.]培养料后发酵过程中的细菌群落结构进行研究。细菌16S rDNA文库ARDRA分析结果表明,细菌群落在后发酵过程中发生了明显变化,4个时期文库的16S rDNA组成分别以各自独有酶切类型为主。ARDRA文库优势类群的序列分析结果显示,培养料中的细菌组成远多于传统分离方法所获得的类群:在"空气调节"时期培养料(C6)中发现了一些未曾报道的Bacilli门成员,如Trichococcus属、Planococcus属和Caryophanon属,以及γ-Proteobacteria亚纲;而在"后发酵"开始和结束的培养料(C1和C7)中分别检测到了Ther mus ther mophilus和α-Proteobacteria亚纲的细菌,这些是近年来利用分子生物学方法在高温堆肥中发现的新类群;同时,在整个"后发酵"过程中还发现了大量目前尚未获得培养的细菌类群。
作者 何丽鸿 于荣利 陈明杰 潘迎捷
机构地区 中华人民共和国农业部应用真菌资源与利用重点开放实验室上海市食用菌工程技术研究中心上海市农业遗传育种重点实验室上海市农业科学院食用菌研究所 南京农业大学生命科学院微生物系 上海海洋大学
出处 《食用菌学报》 北大核心 2009年第1期1-5,共5页 Acta Edulis Fungi
基金 农业部公益性行业科研专项项目“食用菌菌种质量评价与菌种信息系统研究与建立”(编号:nyzx-07-008)的部分研究内容
关键词 双孢蘑菇 培养料后发酵 细菌16S RDNA 细菌群落结构 ARDRA
分类号 S646 [农业科学—蔬菜学]
  • 相关文献

参考文献7

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共引文献4

同被引文献28

  • 1迟桂荣.杏鲍菇多糖对鸡群免疫功能调节的研究[J].安徽农业科学,2007,35(15):4536-4536. 被引量：18
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食用菌学报
2009年 第1期

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