摘要
The Box-Behnken design was used to determine the optimal time,temperature and solvent∶solid ratio for extracting intracellular polysaccharide from G.frondosa mycelium produced under submerged culture conditions.Application of response surface methodology predicted a maximum polysaccharide extraction yield of 7.28% using an extraction time of 2.24 h,an extraction temperature of 92.53 ℃,and a water∶mycelium ratio of 35.76∶1.Under these conditions,the actual yield was 7.15%.Both low(200 mg/kg/d)and high(500 mg/kg/d)doses of G.frondosa polysaccharide,and positive controls administered 20 mg/kg/d cyclophosphamide,significantly inhibited the growth of S180 tumors with inhibition values of 61.5%,44.42% and 73.8%,respectively compared with negative controls administered physiological saline.
The Box-Behnken design was used to determine the optimal time, temperature and solvent ： solid ratio for extracting intracellular polysaccharide from G. frondosa mycelium produced under submerged culture conditions. Application of response surface methodology predicted a maximum polysaccharide extraction yield of 7.28% using an extraction time of 2.24 h, an extraction temperature of 92.53℃, and a water ： mycelium ratio of 35.76 ： 1. Under these conditions, the actual yield was 7.15%. Both low （200 mg/kg/d） and high （500 mg/kg/d） doses of G. frondosa polysaccharide, and positive controls administered 20 mg/kg/d cyclophosphamide, significantly inhibited the growth of S180 tumors with inhibition values of 61.5%, 44.42% and 73.8% , respectively compared with negative controls administered physiological saline.
出处
《食用菌学报》
北大核心
2009年第1期55-58,共4页
Acta Edulis Fungi
基金
Sponsored by the Government of Shandong Province(No.2007LZ07)
关键词
菌丝
生理盐水
灰树花
栽培技术
Grifola frondosa
intracellular polysaccharide
Box-Behnken design
S180 tumor
