牛蒡不同部位、牛蒡子不同产地中牛蒡苷和牛蒡苷元含量比较及指纹图谱分析

Content Determination of Arctiin and Arctigenin in Fructus Arctii from Different Cultivation Places and the Different Parts of Arctium Lappa L. and Fingerprint Analysis of Fructus Arctii

目的:应用HPLC同时测定采集于全国34份牛蒡子和牛蒡不同部位中牛蒡苷和牛蒡苷元的含量;建立牛蒡子药材HPLC指纹图谱。方法:采用AgilentTC-C18(4.6mm×250mm,5μm)色谱柱;流动相:甲醇-水(水:0min,55%;10min,46%;20min,30%线性梯度),检测波长为280nm。结果:牛蒡苷的线性方程为Y=540.83X-937.02(r=0.9999),n=6,平均回收率为97.86%,RSD=1.58%;牛蒡苷元的线性方程为Y=38.257X-24.926(r=0.9998),n=6,平均回收率为98.33%,RSD=1.74%。本实验建立的牛蒡子药材HPLC指纹图谱相似度均在0.90～1.00。结论:本方法简便、准确、灵敏,可同时测定牛蒡苷和牛蒡苷元的含量,可用作牛蒡子药材的质量控制方法。本实验建立的HPLC指纹图谱分析方法可用于评价牛蒡子药材的内在质量。牛蒡不同部位所含牛蒡苷和牛蒡苷元考察结果表明,牛蒡绒毛、果皮、总苞、芽较其他部位含有较多牛蒡苷和牛蒡苷元。

Objective： To establish a HPLC method to determine the contents of aretiin and arctigenin in Fructus Arctii from different cultivation places and the different parts of Arctium Lappa L. and fingerprint of Fructus Arctii. Methods： The HPLC method was employed by taking Agilent TC-C18 scolumn （4.6mm × 250mm, 5μm） and methanol-water （45：55 to 70：30 linear gradient） as mobile phase with detection wavelength at 280 nm. Results： The average recoveries of arctiin and arctigenin are 97.86% and 98.33% respectively. The linear equations were Y = 540.83X - 937.02 （ r = 0. 999 9） and Y = 38. 257X - 24. 926 （ r = 0. 999 8 ） . The similarity of HPLC fingerprint was between 0. 90 and 1.00. Conclusion： The HPLC method is simple, accurate and sensitive. It can be used for determination of Arctiin and Arctigenin of Fructus Arctii. The results indicate that the contents of villus, pericarp, involucre, bud of Arctium lappa L. are higher than the other parts. The HPLC fingerprint method can be used for quality control of Fructus Arctii.