nm23基因与结核菌L型感染在前列腺癌中的表达及意义

Expression of nm23 gene and L-type tuberculosis infection in prostate carcinoma and their significance

目的探讨转移抑制基因nm23和结核菌L型感染在前列腺癌中的表达及临床相关性研究。方法应用免疫组化、原位杂交和抗酸染色等方法检测了65例前列腺癌（carcinoma of prostate，Pea）中的nm23蛋白及mRNA的表达，以及结核菌L型的检出率。并对前列腺癌主要临床资料和病理分级参数进行比较，用χ^2检验进行统计学处理。结果nm23蛋白及mRNA阳性表达与前列腺癌的临床分期、病理分级差异有显著性（P〈0．01～0．05）；无淋巴结转移者高于有淋巴结者，2组之间差异有显著性（P〈0．05），呈负相关。结核菌L型检出率与前列腺癌的临床分期、病理分级差异有显著性（P〈0．05）；有淋巴结转移者明显高于无淋巴结转移者，2组之间差异有显著性（P〈0．05），呈正相关。结论nm23蛋白及mRNA在前列腺肿瘤中有不同程度的异常表达，可能与结核菌L型感染有协同致瘤作用。提示nm23基因可作为判断前列腺癌分化程度及患者预后参考指标。结核菌L型感染与前列腺癌的发生发展可能有一定关系，因此研究结核菌L型感染与前列腺癌的关系，具有重要的临床应用价值。

Objective To explore the expression of metastasis suppressor gene nm2.3 and L-type of tuberculosis infection in prostate cancer and the clinical correlation between them. Method Immunohistochemistry, in situ hybridization and acid-fast stain were used to determine the expression of nm23 protein and mRNA as well as the detection rate of L-type tuberculosis in 65 cases of prostate cancer （Pea）. The clinical data and pathological classification parameters of prostate cancer were analyzed with χ^2 test. Result There were significant differences between each clinical stage of prostate cancer in regard to the positive expressions of mn23 protein and mRNA （P 〈 0.01 - 0.05） ; the positive rate of patients without lymph node metastasis were higher than that of patients with lymph nodes metastasis, and the difference between the two groups was significant （P 〈 0.05 ） with a negative correlation. As to L-TB detection rates, the differences between each clinical stage of prostate cancer were significant （P 〈 0.05 ） ; the positive rate of patients with metastasis were significantly higher than those without metastasis, and the difference between the two groups was significant （P 〈 0.05 ） with a positive correlation. Conclusion Both nm23 protein and mRNA had abnormal expression in prostate cancer and might have a synergistic effect with L-form tuberculosis infection in tumor initiation,which implies that nm23 gene can be used as a reference to evaluate the differentiation of prostate cancer and prognosis of patients. L-form myeobacterium tuberculosis infection might have a certain relation with the development of prostate cancer,the study on which could have an important clinical value.