花生成熟胚叶体细胞胚形成过程中的过氧化物酶同工酶研究

Study on Peroxidase Isozymes During Somatic Embryogenesis from Leaflets of Mature Peanut(Arachis hypogaea L) Embryos

以花生成熟胚的幼叶做外植体诱导体细胞胚发生．从外植体培养到子叶胚形成期间，可溶性蛋白浓度初期下降，以后相对稳定；过氧化物酶活性逐渐升高，过氧化物同工酶谱带由２条陆续增加到１０条，球形胚形成以后同工酶谱带不再变化．

Somatic embryos are induced from leaflets of mature peanut(Arachis hypogaea L.) embryos.Biochemical study reveals that soluble protein concentration maintains a relatively steady level after an original decline while peroxidase activities increases gradually during the process of somatic embryogenesis.Peroxidase isozyme sprectrum increases both in the number of spectrum bands and in intensity during the early period of culture.The number of spectrum bands increases gradually from two of the 0day explants to ten of the globularstaged embryos,and thereafter the sprectrum remains the same until the cotyledonal embryo stage.