短果杜鹃组培快繁及其种质试管保存培养基的筛选

Culture Media for Micropropagation and Germplasm Preservation in Vitro of Rhododendron brachycarpum

以短果杜鹃新生嫩芽为外植体,应用均匀设计法筛选其基部直接再生芽苗、生根及其试管苗保存培养基,结果表明,最适合的基部直接再生芽苗诱导培养基为:DR+2-ip2.75mg.L-1+IAA0.07mg.L-1;生根培养基:1/2MS(大量元素)+IAA0.1mg.L-1+NAA0.05mg.L-1+Kt0.2mg.L-1;试管苗保存培养基:1/5MS+根皮苷5.0mg.L-1。短果杜鹃组织培养的不同阶段需要不同的培养基,常温条件下,利用"低营养延缓生长"的方法在试管内保存其种质。

The most suitable media for differentiation, rooting and germplasm preservation in vitro were screened out by uniform design using tender buds of Rhododendron brachycarpum D. Don as explants. The optimal culture media were obtained as DR supplemented with 2-ip 2.75mg·L^-1 and IAA 0.07 mg·L^-1for callus differentiation, 1/2MS with IAA 0.1 mg·L^-1, NAA 0.05 mg·L^-1 and Kt 0.2 mg·L^-1 for rooting, and 1/5 MS with phlorizin 5.0 mg·L^-1 for germplasm preservation in vitro. Tissue culture of R. brachycarpum required different kinds of culture media in different phases. The method of deferring growth with low nutrients was applied to germplasm preservation in vitro at normal temperature.