系统性红斑狼疮患者外周血单一核细胞CCR6、CCR8趋化因子受体mRNA的表达

Expression of chemokine receptors (CCR6 and CCR8 ) mRNA in patients with SLE

目的:探讨趋化因子受体CCR6及CCR8在系统性红斑狼疮(SLE)患者外周血单一核细胞(PBMC)中的表达及与疾病的相关性。方法:收集93例确诊的SLE患者和30例正常对照者的PBMC,提取RNA。应用RT-PCR方法检测研究对象CCR6、CCR8 mRNA表达水平。记录患者临床表现及实验室检查结果。结果:①CCR6 mRNA在PBMC中表达水平:活动期组(0.985±0.257)与对照组(0.229±0.041)相比,两者差异有统计学意义(t=2.910,P=0.006)。活动期组与非活动期组(0.306±0.034)、非活动期组与对照组、患者组(0.641±0.179)与对照组,3组间差异均无统计学意义(P>0.05)。CCR8 mRNA在PBMC中表达水平:活动期组(0.703±0.285)与非活动期组(0.219±0.031)及对照组(0.120±0.018)间比较,差异无统计学意义(P>0.05);非活动期组与对照组、患者组(0.549±0.663)与对照组,两组间差异无统计学意义(P>0.05)。②患者组CCR6 mRNA水平与疾病活动度评分关系:SLE患者组PBMC中CCR6 mRNA表达水平与SLE疾病活动程度指数(SLEDAI)分别作直线相关性分析,CCR6 mRNA水平与SLEDAI(r=0.457,t=4.513,P<0.001)呈正相关。③患者分为两个亚组:具有某些临床表现的与不具临床表现的CCR6mRNA及CCR8 mRNA表达,差异无统计学意义。结论:CCR6 mRNA表达水平在活动期SLE患者比非活动期及对照组增高,与SLEDAI呈正相关。CCR8 mRNA表达水平在SLE患者组与正常对照组差异无统计学意义。CCR6可能参与SLE的发生。

Objective： To investigate the expression of chemokine receptors in the patients with systemic lupus erythematosus （SLE） and its correlation to SLE disease activity index （SLEDAI）. Methods： The mRNA expression of CCR6 and CCR8 on the peripheral blood mononuelear cells（PBMC） of 93 SLE patients and 30 healthy controls were detected by reverse transcription-polymerase chain reaction （RT-PCR）. Results： The mRNA level of CCR6 in patients with SLE （0.985±0.257）was significantly higher than that of normal controls （0.229±0.041）（t = 2.910,P= 0.006）, with positive correlation to the SLEDAI. There was no difference in the level of CCR8 expression. Conclusions： These results suggest that CCR6 might be involved in the pathogenesis of SLE, and the CCR6 level might be a good indicator for the disease activity of SLE.