摘要
生理和生化实验结果表明Fe(Ⅲ)-EDTA是SF3偏好的一种可溶性三价铁.连二亚硫酸盐还原后的SF3经过可溶性三价铁氧化后,其紫外可见光谱显示c型细胞色素参与了三价铁的还原过程.铁还原酶的定位实验说明87%的铁还原酶活性定位于细胞可溶性组分,74%的活性分布于原生质体中,这种分布情况可能与一种和其他细菌不同的铁还原机制有关.SF3的铁还原酶在25℃,pH7.4时显示最高活性,并且在氧气中表现出较强的稳定性.
Both physiological and biochemical studies have demonstrate that Fe （Ⅲ）-EDTA is a favorite soluble Fe（ Ⅱ ） of strain SF3. The UV-visible spectra of dithionite-reduced, soluble Fe （Ⅲ ） reoxidized cell extracts indicates the involvement of c-type cytochromes in Fe（ Ⅲ ） reduction. Fractionation experiments suggest that 87% of the Fe（ Ⅲ） reducing activity is localized at soluble fraction, 74% of the Fe（Ⅲ）-reducing activity is found in the spheroplasts, which may indicate a distinct mechanism for Fe （ Ⅲ ） reduction. Fe （Ⅲ ） reductase of strain SF3 exhibits the highest activity at 25 ℃, pH 7.4, and shows a great oxygen-tolerance ability.
基金
Supported by"100 Talents Program"of Chinese Academy of Sciences
National Natural Science Foundation of China (30470943)
