摘要
TD1作为一种含有11个氨基酸的短肽,具有良好的促进蛋白类大分子透皮的功能.过去的研究显示TD1可以有效协助胰岛素通过皮肤进入循环并最终降低血糖.在本研究中我们构建了一种TD1 N端修饰的GFP融合蛋白(TGFP).我们的实验表明,与TD1与GFP蛋白的混合物相比,TGFP具有更加良好的透皮功能.这一发现为透皮给药研究提供了一条新的途径,并对解释TD1透皮功能具有指导意义.
TD1 peptide, with the amino acid sequence of ACSSSPSKHCG, has been reported to have the potential ability to facilitate transdermal delivery of macromolecules such as insulin through skin. Here we report that TD1-GGS-eGFP （TGFP）, or the N-terminal TD1 modified green fluorescent protein （ GFP）, transporting through skin barrier in provide a new way for transdermal methodology for the elucidation of transdermal delivery. compared to the unmodified GFP, is more capable of comparison with the unmodified GFP. This finding may drug delivery. It can also offer a new experimental the mechanism through which TD1 peptide facilitates
基金
Supported by National basic research program of China,also called 973 program(2006CB933300)
The cultivation fund of key scientific and technical innovation project, ministry of education of China (706035)
