HPLC同时测定通窍益心丸中华蟾酥毒基和脂蟾毒配基的含量

Determination of Cinobufagin and Recibufogenin in Tongqiao Yixin Pill by HPLC

目的建立测定通窍益心丸中华蟾酥毒基和脂蟾毒配基含量的反相高效液相色谱法。方法采用Elite SinoChromODS2色谱柱,以0.5%磷酸二氢钾溶液(pH3.2)-乙腈(52∶48)为流动相,流速:1.0 mL.min-1,检测波长:296 nm。结果华蟾酥毒基回收率99.77%,RSD=0.49%;脂蟾毒配基回收率99.39%,RSD=0.60%。结论本法简便准确,专属性强,可用于控制该制剂质量。

OBJECTIVE To establish an HPLC method for the determination of cinobufagin and recibufogenin in Tongqiao Yixin pill. METHODS The analysis was performed on a SinoChrom ODS2 column. The mobile phase consisted of 0.5% potassium phosphate（ pH 3.2） -acetonitrile （52： 48） , and the flow rate was 1. 0 mL · min^-1. The detection wavelength was 296 nm. RESULTS The average recovery, and RSD were 99.77% and 0.49% for cinobufagin, 99.39% and 0.60% for recibufogenin, respectively. CONCLUSION This method is simple and reliable for the quality control of Tongqiao Yixin pills.