摘要
目的探讨中国汉族人群散发性Alzheimer病(SAD)患者脑源性神经营养因子(BDNF)基因C270T多态性与AD发病的关系。方法采用聚合酶链反应限制性片段长度多态性(PCR-RFLP)技术,检测55例SAD患者和80名年龄、性别匹配的健康人(正常对照组)BDNF基因C270T多态性,比较两组基因型分布和等位基因频率。结果正常对照组BDNFC270T基因型及等位基因频率分布符合Hardy-Weinberg定律(χ2=0.167,P=0.682),SAD组基因型[C/C型52例(94.5%),C/T型3例(5.5%)]及等位基因频率(C97.27%,T2.73%)分布与正常对照组[C/C型74例(92.5%),C/T型6例(7.5%);C96.25%,T3.75%]比较差异无统计学意义。结论中国汉族人群BDNF基因C270T多态性与SAD的发病无明显关系。
Objective To investigate the association between the polymorphisms of brain-derived neurotrophic factor (BDNF) gene C270T and sporadic Alzheimer's disease (SAD) in Han Chinese. Methods The polymerase chain reaction-restriction fragment length polymorphism (PCR-RELP) was used to detect BDNF gene C270T genotype and allele frequencies in 55 SAD patients and 80 age- and sex-matched healthy people( as normal control group). Results The frequencies distribution of BDNF gene C270T genotype and allele in the normal control group were suit to Hardy-Weinberg equilibrium (χ^2 = 0. 167, P = 0.682). The frequencies distribution of genotype and allele in the SAD group[ C/C:52(94.5% ), C/T:3(5.5% ) and C:97.27%, T:2.73% ] and normal control group[ C/C:74 (92.5%), C/T:6(7.5% ) and C :96.25%, T:3.75% ] were no statistical differences. Conclusion There is no association between the polymorphisms of BDNF gene C270T and SAD in Han Chinese.
出处
《临床神经病学杂志》
CAS
北大核心
2009年第2期90-92,共3页
Journal of Clinical Neurology
基金
广东省自然科学基金自由申请项目(05001277)