摘要
microRNA是真核生物中一大类参与基因转录后水平调控的长度为20 ̄24个核苷酸的非编码小分子RNA,它们在生物体的细胞增殖、细胞分化、细胞凋亡、形态建成、个体发育、激素分泌与信号转导等一系列生命活动中起重要作用。现以小鼠调控过氧化物酶体生物合成和增殖的蛋白质基因为靶基因,采用生物信息学方法搜寻到了调控这些靶基因表达且在系统发育上保守的microRNA,为进一步鉴定参与过氧化物酶体生物合成和增殖的microRNA,阐明microRNA在过氧化物酶体的生物合成和增殖中的作用和调控机制提供有价值的信息。
MicroRNAs are small non-coding RNAs with 20-24 nucleotides in length and believed to be involved in a broad range of biological function, including cell proliferation, cell differentiation, cell apoptosis, development, responses to diseases and signal transduction, by base-pairing with complementary sites of target transcript to negatively regulate gene expression at the posttranscriptional level. Here we used a bioinformatics approach to identify conserved microRNAs which can complement with 3′ untranslation region of target transcripts involved in peroxisome biogenesis and proliferation.
出处
《细胞生物学杂志》
CAS
CSCD
2009年第2期257-264,共8页
Chinese Journal of Cell Biology
基金
国家自然科学基金(No.30660042)
江西省自然科学基金(No.0630137)资助项目~~