骨桥蛋白在不同转移潜能肝癌细胞系及肝癌组织中表达意义

Osteopontin expression in hepatocellular carcinoma cell lines with different metastatic potential and HCC samples

目的研究不同转移潜能人肝癌细胞系及其肝癌组织中骨桥蛋白（OPN）表达情况，分析OPN表达与肝癌细胞侵袭转移潜能、肝癌术后复发转移的关系。方法应用细胞免疫组化、半定量RT-PCR、Western Blot和ELISA检测不同转移潜能人肝癌细胞系SMMC7721、MHCC97-L和HCCLM6OPN表达情况。应用组织芯片、免疫组化检测200例肝癌组织中OPN表达。结果OPN表达水平随着肝癌细胞侵袭转移潜能增加逐渐升高。肝癌组织中0PN表达与病人性别、年龄、血清AFP水平、肿瘤大小、有无包膜、细胞分化以及肝门淋巴结转移无明显相关性，而与肝癌TNM分期、门静脉癌栓和术后复发转移发生有明显相关（P〈0．05）。术后有复发转移病人的肝癌组织中66％表达OPN，而术后无复发转移病人的肝癌组织中仅37％表达OPN，两者比较差异具有显著性（P〈0．05）。结论OPN表达与肝癌细胞细胞系侵袭转移潜能密切相关。肝癌组织中OPN表达可能是预测肝癌术后是否发生复发转移指标。

Objective To study the relationship between OPN expression and HCC cell line metastatic potential and HCC recurrence/metastasis after resection. Methods Immunocytochemistry, RT-PCR, Western Blot and ELISA were applied to detect OPN expression of HCC cell lines SMMC7721, MHCC97-L and HCCLM6 with different metastastic potential. Immunohistochemistry of OPN was performed in the tissue arrays containing 200 HCC samples. Results The OPN expression was leveled with increasing metastatic potential of cell lines. OPN expression of samples was not associated with age, sex, serum AFP level, tumor size, cell differentiation, tumor capsule and lymphatic node metastasis but significantly associated with HCC TNM classification, portal vein tumor thrombi and HCC recurrence/metastasis after resection. OPN expression detected in 66% of HCC samples of patients with recurrence/metastasis after resection was significantly higher than 37% of the samples of patients without recurrence/metastasis after resection （P〈0. 05）. Conclusion OPN expression is associated with HCC cell line metastatic potential and maybe an indicator of HCC recurrence/metastasis after resection.