食管癌病人外周血清中微卫星杂合缺失的检测及其意义

Significance of loss of heterozygosity with microsatellite in the esophageal cancer

目的探讨食管癌病人肿瘤组织中存在的微卫星变化是否可在血清中检出，以及血清样本的微卫星分析在临床应用的可行性。方法采用PCR荧光测序仪凝胶电泳分别检测68例食管癌病人手术切除的鳞癌标本及其匹配的血清样本中13个微卫星位点的杂合性缺失（LOH）状况，同时以100位健康成人的血清样本作为正常对照；统计比较分析LOH与临床病理参数之间的关系。结果68例病人中有62例肿瘤样本检出至少1个位点以上的杂合性缺失，有64例血清样本检出至少1个位点以上的杂合性缺失；两种样本中13个微卫星缺失的总体检出阳性率与食管癌的临床分期没有显著相关性；发生转移的病人血清LOH检出率较未发生转移者偏高；分化差的病人血清LOH检出率较分化好者偏高。结论通过血清样本的微卫星分析可判定食管癌病人恶性肿瘤的存在；血清微卫星分析将有助于高危病人早期食管癌的检出。

Objective To investigate whether the loss of heterozygosity of microsatellite present in tumor tissues of patients with esophageal cancer could be detected in the sentm sample, and its clinical significance. Methods 68 tumor specimens and their matched serum DNAs were analyzed for LOH at 13 microsatellites by using PCR and fluorescence-based DNA sequencing technology. Serum samples from 100 healthy persons served as normal controls, The association of LOH with the clinicopathological phenotype was compared. Results 64（94.1% ） patients（94.1% ） were detected to have LOH at least one microsatellite site in tumor tissue, and 66（97.0% ） in serum samples. The detection rate for LOH was not significantly associated with the clinical stage of esophageal can- cer. There was a tendency toward more frequent LOH in the sermn in patients with lymph node involvement and higher histological grade. Conclusion The presence of tumor can be determined by microsatellite analysis in the serum of patients with esphageal cancer, andsertan microsatellite anaysis will be hilpful of the early detection of esophageal cancer in high-risk populations.