4-氨基-2-三氟甲基苯基维甲酸酯诱导的肺癌细胞的RARα、EGFR转录水平的变化

Effects of 4-amino-2-trifluoromethyl-phenyl retinoate on expression of RARα and EGFR in lung cancer cells

目的探讨4-氨基-2-三氟甲基苯基维甲酸酯对肺癌细胞RARα、表皮生长因子受体(EGFR)表达的影响。方法用4-氨基-2-三氟甲基苯基维甲酸酯处理肺癌细胞24、48、72h后,用RT-PCR方法分析细胞RARα、EGFR表达的变化。结果4-氨基-2-三氟甲基苯基维甲酸酯作用于肺癌细胞24、48、72后,A549和L78细胞的EGFR表达下降显著,并呈时间依赖(P<0.01);A549和L78细胞的RARα表达增加并呈时间依赖(P<0.05)。结论4-氨基-2-三氟甲基苯基维甲酸酯可以显著降低肺癌细胞EGFR的表达,同时显著增加肺鳞癌细胞RARα表达。

Objective To study the effect of 4-amino-2-trifluoromethyl-phenyl retinoate on expression of RARα and EGFR in lung cancer ceils. Methods 4-amino-2-trifluoromethyl-phenyl retinoate was used to treat lung cancer cells for 24,48,72 h; RT-PCR was used to analyze the expression changes of RARα and EGFR before and after treatment with 4-amino-2-trifluoromethyl-phenyl retinoate in lung cancer cells. Results The expression of EGFR in A549 and L78 cells was decreased （P 〈0. 01 ） and the expression of RARer in 3.549 and L78 cells was increased （P 〈 0. 05 ） after treatment with 4-amino-2-trifluoromethyl-phenyl retinoate. Conclusion The expression of EGFR in A549 and L78 cells are inhibited by 4-amino-2-trifluoromethyl-phenyl retinoate.