甲状腺癌组织VEGF-C和nm23表达及其临床意义的探讨

Expressions and significance of VEGF-C and nm23 in thyroid cancer tissues

目的:研究VEGF-C和nm23在甲状腺癌组织中的表达及相互关系,探讨其临床意义。方法:用免疫组化EnvisionTM二步法检测63例甲状腺癌组织、10例癌旁组织和10例结节性甲状腺肿组织中VEGF-C和nm23的表达,结合临床病理因素进行分析。结果:VEGF-C蛋白在甲状腺癌组织中的表达显著高于在结节性甲状腺肿和癌旁甲状腺组织中的表达,P<0.01;VEGF-C蛋白的阳性率与淋巴结有无转移相关,P<0.01。nm23蛋白在甲状腺癌组织中的表达与年龄、淋巴结转移及临床分期有关,P<0.05。VEGF-C与nm23在甲状腺癌中表达负相关,r=-0.592,P<0.01;VEGF-C阳性表达伴nm23阴性表达组的甲状腺癌淋巴结转移率显著高于VEGF-C阴性表达伴nm23阳性表达组,P<0.01。结论:VEGF-C在甲状腺癌组织中高表达,与甲状腺癌的侵袭和转移正相关,nm23与甲状腺癌的侵袭和转移负相关。两者在甲状腺癌组织中的表达负相关,联合检测VEGF-C和nm23可能有助于甲状腺癌淋巴结转移的判断。

OBJECTIVE： To investigate the expressions of vascu lar endothelial growth factor-C（VEGF-C） and non-metastasis（nm23） in thyroid cancer tissues and their relationship, and explore their clinical value. METHODS： The paraffin-embedded tissue sections of 63 thyroid cancer patients, 10 eases of multinodular goiter and 10 cases from the peritumor tissue were immunohistochemically examined for the expressions of VEGF-C and nm23. The association between VEGF-C and nm23 expressions and clinicopathological parameters were analysised. RESULTS.. The positive rate of VEGF-C in thyroid cancer tissues was significantly higher than those in multinodular goiter and peritumor tis sues, P〈0.01. The positive expression of VEGF-C was correlated significantly with the lymph node metastasis, P〈0.01. The positive rate of nm23 in thyroid cancer tissues was correlated significantly with age, lymph node metastasis and clinical stage, P〈0.05. A significant reverse correlation was found between the expressions of VEGF-C and nm23 in thyroid cancer, r=-0.592, P〈0.01. Furthermore,the rate of lymph node metastasis in thyroid cancer of VEGF-C positive with nm23 negative was significantly higher than that in VEGF-C negative with nm23 positive, P〈0.01. CONCLUSIONS： The VEGF-C is overex pressed in thyroid cancer tissues, and associated with metastasis and invasion of thyroid cancer. A significant negative correlation is found in thyroid cancer between the expression of nm23 and capability of metastasis and invasion. There is a significant reverse correlations between the expression of VEGF C and nm23 in thyroid cancer. VEGF-C and nm23 are beneficial to diagnosis of cervical lymph node metastases in thyroid cancer.