荧光原位杂交检测乳腺癌HER-2基因的应用价值

A Method for Detection of HER-2 Gene Amplification in Breast Cancer Tissues: Fluorescence in Situ Hybridization Method And its Application

目的:探讨荧光原位杂交(FISH)在检测乳腺癌组织HER-2基因扩增的应用价值。方法:收集50例手术切除的乳腺癌标本,经过处理和切片,FISH法检测细胞HER-2基因扩增情况,IHC法检测细胞膜上HER-2蛋白表达情况。kappa检验法对两种检测结果的一致性进行统计分析。结果:FISH法与IHC法检测结果总符合率为90%(K=0.75,P<0.05)。在本组胶东半岛女性乳腺癌人群中HER-2基因扩增率为28%。结论:FISH法检测乳腺癌HER-2基因敏感性及稳定性较高,可作为最终确诊HER-2基因状态的方法。

Objective： To evaluate the application value of fluorescence in situ hybridization （FISH） in detecting HER2 gene amplification of breast cancer tissue. Methods： Cancer tissue samples were collected from 50 women with breast cancer. IHC technique was applied to detect the expression of Her-2 protein in the cytomembrane of breast cancer, and FISH was used to assess the amplification of Her-2/neu gene. Kappa test was employed to compare the results by the two methods. Results： The coincident rate of the two methods was 90 %, therefore there was a significant positive correlation between the two techniques （K=0.75, P〈0.05）. The amplification rate of HER-2 gene is 28 % in the tested group. Conclusion： FISH had good sensitivity and stability in detecting HER2 gene amplification of breast cancer tissue, thus could be conducted to diagnose HER2 gene.