摘要
目的:探讨诱骗受体3(DCR3/TR6)在贲门癌组织中表达及其临床病理意义。方法:采用二步法免疫组化技术(Envision)检测66例贲门癌组织中DCR3/TR6的表达情况,分析DCR3/TR6与各临床病理因素的关系。结果:贲门癌组织DCR3/TR6阳性表达率为54.5%(36/66),癌旁正常胃黏膜无DCR3/TR6表达,两者之间差异有统计学意义,P=0.000。DCR3/TR6蛋白在低分化贲门癌中表达为69.0%(29/42),与高、中分化癌的29.2%(7/24)相比,差异有统计学意义,χ2=8.255,P=0.004。在伴有淋巴结转移者表达为68.9%(31/45),无淋巴结转移者表达为23.8%(5/21),差异有统计学意义,χ2=9.988,P=0.002。伴有浆膜层浸润者表达为69.2%(27/39),无浆膜层浸润者表达为33.3%(9/27),差异有统计学意义,χ2=6.908,P=0.009。TNM分期Ⅲ期的贲门癌DCR3/TR6阳性表达率90%(18/20)显著高于Ⅰ、Ⅱ期的39.1%(18/46),χ2=12.569,P=0.000。DCR3/TR6基因表达与贲门癌组织的分化程度、临床分期及淋巴结转移呈正相关,而与贲门癌的年龄、性别及肿瘤大小无相关性。结论:通过阻断凋亡,DCR3/TR6可能促进贲门癌细胞生长,检测DCR3/TR6蛋白表达对于判断贲门癌的恶性程度及病情进展有一定的价值。
OBJECTIVE: To explore the the expression of decep tive receptor-3(DCR3/TR6)in the tissue of cardiac cancer and its clin ical significance. METHODS: Two-step immunohistochemistry technology was adopted to detect the expression of deceptive receptor-3 (DCR3/TR6)in the tissue of cardiac cancer and the relation of the expression to clinicopathologieal features of cardiac cancer was analysed. RESULTS:The positive expression rate of DCR3/TR6 in cardiac canc er tissue was 54.5%(36/66), meanwhile the expression was not found in normal gastric mucosa, suggesting a significant difference between both, P = 0. 000. Compared with the DCR3/TR6 protein positive expression rate of 29.2 %(7/24) in moderate and high differentiated cardiac cancer tissue, the DCR3/TR6 protein positive expression rate of of 69.0% in low differentiated cardiac cancer tissue (29/42) presented statistical significance,χ^2=8. 255,P=0. 004. The DCR3/TR6 positive expression rate was 68. 9%(31/45) in the pa tients with lymph nodes metastasis, significantly higher than that of 23.8%(5/21) in the patients without lymph nodes metastasis, ;(χ^2=9. 988,P=0. 002. In the meantime, The DCR3/TR6 positive expres sion rate of 69.2%, (27/39) in the patients with serosal infiltration was also significantly higher than that of 33.3% (9/27) in the patients without serosal infiltration, χ^2=6. 908, P = 0. 009. The DCR3/TR6 protein positive expression rate in TNM classification stage Ⅲ cardinc cancer tissue was 90.0G (18/20), significantly higher than that of 39. 1%(18/46) in stage Ⅰ and stage Ⅱ cardiac cacer, χ^2=12.569,P= 0.000. The DCR3/TR6 gene expression was positively related to the tumor differentiation, clinical stages and lymph nodes metastasis, but not to the age, sex and tumor size. CONCLUSIONS: Through blockade of FasL, DCR3/TR6 may promote tumor growth of cardiac cancer. The detection of DCR3/TR6 protein expression can be worthwhile to judge the malignance and progression of cardiac cancer.
出处
《中华肿瘤防治杂志》
CAS
2009年第5期365-367,共3页
Chinese Journal of Cancer Prevention and Treatment
基金
广东省医学科研基金资助项目(A2007746)
关键词
胃肿瘤
贲门
受体
肿瘤坏死因子
免疫组织化学
stomach neoplasms
cardia
receptors, tumor necrosis factor
immunohistochemistry