生地对广防己减毒作用最佳配伍比例的研究

Study on Best Comparability Ratio of Radix Rehmanniae with Radix Aristolochiae Fangchi for Attenuation of Toxicity

目的通过测定生地对广防己中马兜铃酸A含量的影响,初步研究生地对广防己减毒作用的最佳配伍比例。方法广防己与生地配伍以95%的乙醇超声提取后,用反相高效液相色谱法测定马兜铃酸A的含量,并观察含量的变化情况。结果加入不同配比的生地后,广防己中马兜铃酸A的含量均降低,其中以广防己∶生地=2∶1的比例最为明显。结论生地可降低广防己中马兜铃酸A的含量,具有减毒作用,以广防己∶生地=2∶1为最佳配伍比例。

Objective To study initially the best comparability ratio of Radix Rehmanniae with Radix Aristolochiae Fangchi for attenuation of toxicity through determination of the content of aristolochia acid A in Radix Aristolochiae Fangchi.Methods Radix Aristolochiae Fangchi is combined with Radix Rehmanniae and extracted ultrasonically with 95% ethanol.RP-HPLC method was used to determine the content of aristolochhia acid A and observe its changes.Results With Radix Rehmanniae of different ratios modified,the content of aristolochia acid A in Radix Aristolochiae Fangchi was all reduced, which was the most apparent at the ratio of 2：1 between Radix Aristolochiae Fangchi and Radix Rehmanniae. Conclusion Radix Rehmanniae reduces the content of aristolochia acid A in Radix Aristolochiae Fangchi and presents its attenuation. The ratio 2：1 is the best comparability of Radix Rehmanniae with Radix Aristolochiae Fangchi for attenuation of toxicity.