腹泻住院患儿中检测到人双埃可病毒

Detection of human parechovirus in children hospitalized for acute gastroenteritis

目的在腹泻住院患儿粪便标本中检测人双埃可病毒（HPeV），探讨HPeV与婴幼儿重症腹泻之间的联系。方法通过Real—timePCR直接从〈5岁未知病原住院腹泻患儿粪便标本中检测HPeV，进而通过基于VP3／VP1连接区的巢式PCR扩增片段的测序结果进行分型。结果99份腹泻粪便标本，HPeV阳性24例（24％），其中，以HPeV1为主（50％），其次为HPeV3（25％），HPeV4（8．3％），HPeV6（4．2％），还有3例HPeV阳性标本未能分型，未检测到HPeV2、5、7、8。HPeV感染的住院腹泻患儿主要为〈1岁患儿。HPeV感染高峰为秋冬季节，10月达到峰值。VP3／VP1连接区基因与已知HPeV参考株氨基酸序列同源性均〉98％。结论在中国开展HPeV监测对于进一步阐明我国婴幼儿腹泻的病因构成及深入了解HPeV的病原学及流行病学特点有重要意义。

Objective To detect human parechovirus （HPeV） from stool samples of hospitalized children for acute gastroenteritis of undetectable etiology. Methods We conducted a real-time PCR to detect HPeV. Result The results showed that 24 of 99 （24%） children with gastroenteritis of undetectable etiology were detected with HPeV. Four known HPeV types （HPeV1,3,4, 6） were detected in the present study. HPeV1 （50%） was frequently identified as the predominant strain and follow by HPeV3 （25 % ）, HPeV 4 （ 8.3 % ） and HPeV 6 （4.2 % ）. We were unable to type 3 samples. Conelusion HPeV was prevalent in hospitalized children for aeute gastroenteritis of undetectable etiology in China. Further study is needed for clarifying the role of HPeV in gastroenteritis.