摘要
目的优化BALB／c3T3细胞转化实验，并应用于致癌物间协同致癌作用的研究。方法从血清浓度、培养基类型和致癌物作用时间三个因素对BALB／c3T3细胞转化实验进行优化。采用优化的实验方案，选择致癌物作用7d后重新接种，再以含5％胎牛血清的DMEM／F12（1：1）培养基培养进行细胞转化实验，对致癌物间的协同作用进行检测，通过小鼠体内致瘤实验对转化灶细胞的恶性特征进行验证。结果二乙基亚硝胺（DEN）与2、3、7、8．四氯二苯并二恶英间有较强的协同致癌作用。微囊藻毒素单独具有较强促细胞恶性转化能力，但这种促转化能力却受到DEN的抑制。实验诱发的转化灶具有Ⅱ型转化灶的特征，并可在体液与细胞免疫缺陷（SCID）小鼠体内致瘤。结论经优化的BALB／c3T3细胞转化方案既充分模拟了致癌物联合作用的方式，又缩短了实验周期，可有效应用于致癌物间协同作用的研究。
Objective To improve the protocol of BALB/c 3T3 cell transformation assay, and apply it to the cocarcinogenesis study. Method Appropriate serum concentration, culture media and method of administration were selected by testing their effects on the growth and transformation of BALB/c 3T3 cells. The co-carcinogenic activity between diethylnitrosamine （DEN） and microcystin-LR （MC-LR） or 2,3,7,8-tetrachlorodibellzo-p-dioxin （TCDD） were examined using the improved cell transformation assay. The malignant characteristics of transformed cells were verified by neoplasia in SCID mice. Results There were strong co-carcinogenic activity between DEN and TCDD. On the contrary, although MC-LR has strong ability to induce cell transformation, the effect was markely inhibited by DEN. The transformed cells show some malignant characteristics. Conclusion The improved BALB/c 3T3 cell transformation assay is reliable and time-saving, and can be efficiently used in the study of cocarcinogenesis.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
北大核心
2009年第2期121-123,共3页
Chinese Journal of Experimental and Clinical Virology
基金
基金项目：国家科技支撑计划（No.2006BA119803）
国家自然科学基金（30771812）
