柯萨奇病毒腺病毒受体在非小细胞肺癌中的表达及其意义

Expression of coxsackievirus and adenovirus receptor in non-smaU cell lung cancer and its significance

目的 研究柯萨奇病毒腺病毒受体（CAR）的mRNA和蛋白在正常肺组织、癌旁组织及肺癌组织中的表达情况，分析其与肺癌发生发展的相关性及在不同临床病理参数间的表达差异。方法应用半定量逆转录聚合酶链反应（RT—PCR）方法和Western blot检测正常肺组织、癌旁组织和肺癌组织中CARmRNA和CAR蛋白的表达情况。结果CARmRNA和蛋白在正常肺组织、癌旁组织和肺癌组织中的表达量分别为1．000±0．012、1．048±0．035、1．282±0．072和0．902±0．038、0．944±0．042、1．08±0．052，其差异均有统计学意义（P〈0．05）。CAR mRNA和CAR蛋白在肺癌中的表达呈正相关（r=0．448，P=0．026）。在不同的临床病理参数之间，CAR mRNA和蛋白的表达在患者性别、年龄、吸烟与否、肿瘤大小、有无淋巴结转移及TNM分期中差异无统计学意义（P〉0．05）；CAR的表达在不同的病理类型中差异具有统计学意义（P：0．012），在7例细支气管肺泡癌（BAC）中均高表达（P=0．029）。结论肺癌组织中CAR的表达水平显著高于正常肺组织和癌旁组织，且在不同病理类型的表达上存在差异，提示CAR在肺癌的发生发展过程中起到了重要作用，并可能成为潜在的肿瘤标记及预后指标。

Objective To investigate the mRNA and proten expression of coxsackievirus and adenovirus receptor （CAR） in the corresponding normal lung tissue, para-neoplastic tissue and lung cancer tissue, and the correlation of CAR expression with the carcinogenesis as well as the expression difference in various clinicopathologic parameters. Methods The expression of CAR mRNA and protein in the samples from 32 lung cancer patients was determined by RT-PCR and Western blot, respectively. Results The expression level of CAR mRNA and protein in normal lung tissue, paraneoplastic tissue and cancer tissue were 1. 000 ±0. 012,1. 048± 0. 035,1. 282± 0. 072, and 0. 902± 0. 038,0. 944± 0. 042,1.08 ± 0. 052, respectively, with a statistical significance among the groups （ P = 0. 022, P = 0. 007, P = 0. 009, P = 0. 027 ）. There was a statistically significant positive correlation between expression of CAR mRNA and that of CAR protein （ r = 0. 448, P = 0. 026 ）. The expression levels of CAR were significantly different among different pathological types （ P = 0. 012 ）, with a high level of CAR in all 7 bronchiolo-alveolar carcinoma （BAC, P = 0. 029）. However, there was no statistical significance in other clinicopathologic parameters （P 〉 0.05 ）, including gender, age, smoking or not, tumor size, with or without lymph node metastasis and TNM stage. Conclusion The expression of CAR mRNA and protein in cancer tissue samples are significantly higher than that in the normal and paraneoplastic samples, indicating that CAR might play a crucial role in the carcinogenesis. It may become a new potential prognostic marker for lung cancer patients.