HIF-1α和AAH在稽留流产患者绒毛中的表达研究(英文)

Expression of hypoxia-inducible factor-1α and aspartyl-(asparaginyl) beta-hydroxylase in missed abortion patients' villi

目的研究缺氧诱导因子-1α(HIF-1α)及天冬氨酰(天冬酰胺酰)-β-羟化酶(AAH)在稽留流产患者绒毛中的表达情况,探讨其与稽留流产的关系及意义。方法稽留流产患者50例(研究组),其中有已知可能原因的20例(研究组1),原因不明者30例(研究组2);正常早孕20例作为对照组。研究组50例按胚胎稽留宫内时间长短分为≤4周组和>4周组。应用免疫组织化学方法和计算机图像分析系统检测研究组及对照组绒毛滋养细胞中HIF-1α及AAH的表达。结果HIF-1α及AAH在稽留流产患者及正常早孕妇女绒毛中均有表达,其表达部位主要在滋养细胞的胞质及胞核。研究组1及研究组2绒毛滋养细胞HIF-1α及AAH表达水平均明显低于对照组(P<0.05);但两组间表达水平无显著差异(P>0.05);≤4周组与>4周组之间HIF-1α表达无显著差异(P>0.05)。AAH在稽留流产患者及正常早孕妇女绒毛中均有表达,其表达部位主要在滋养细胞的胞质及胞核。绒毛滋养细胞AAH表达水平在研究组1及研究组2均明显低于对照组(P<0.05);两组间表达水平无显著差异(P>0.05);≤4周组与>4周组之间AAH表达亦无显著差异(P>0.05)。结论HIF-1α及AAH在稽留流产患者绒毛中的表达均低于正常早孕妇女,其低表达可能是导致稽留流产发生的重要因素。

Objective To investigate the expression of hypoxia-inducible factor-1α （HIF-1α） and aspartyl-（asparaginyl） beta-hydroxylase （AAH） in missed abortion patients＇ villi, and explore the relation.ship between these two materials and missed abortion. Methods 50 patients with missed abortion were collected and categorized into two groups （test group 1 and test group 2）. Patients in test group 1 （n=20） were of confirmed etiologies while those in test group 2 （n=30） showed no obviously etiological clues. In addition, 20 early pregnant women with artificial abortion surgery were used as control group, of whom the embryos were confirmed alive before surgery. According to the time that the embryo stayed in uterus after it died, the 50 patients of missed abortion were subdivided into the group of 44 weeks or the group of 〉4 weeks. Immunohistochernical tectmique and computer image analysis system were used to detect the expression loci and level of HIF-1α and AAH in chorionic villi. Results HIF-1α expression was detected in both missed abortion villi （test group） and normal early pregnancy villi （control group）, and its loci were mainly located in endochylema and nucleus of trepbocytes. The expression levels of HIF-1α in test groups were significantly lower than that in control group （P〈0. 05）, but no significant difference existed between the two test groups （P〉 0. 05）. The expression level of HIF-1α in villi from missed abortion women showed no significant differenee between the groups of ≤4 or 〉4 weeks of arrested embryos The AAH expression was found to have the similar result as HIF-1α＇s. Conclusions The expression level of HIF-1α and AAH in villi nf missed abortion patients is much lower than that of normal early pregnant women. HIF-1α and AAH have a function of supporting normal pregnancy, so their low expression may be an important cause of missed abortion.