杀鼠剂溴敌隆和环糊精的超分子作用及分析应用

Supramolecular interaction between rodenticide bromadiolone and cyclodextrin and its analytical application

利用光谱法研究了β-环糊精（β-CD）与杀鼠剂溴敌隆（BRD）的超分子作用，结果发现二者可形成1：1的超分子包络物，室温下包络常数为357.7L／mol。通过相关数据和对4-羟基香豆素的对比试验初步探索了溴敌隆和β-环糊精的包络模式，应该是溴敌隆结构中的疏水基团4-羟基香豆素母环或者溴代联苯基进入了β-环糊精的疏水空腔之中从而形成超分子包络物。实验还观察到这种包络作用可以大大增敏溴敌隆的荧光。据此，建立了水溶液中测定溴敌隆的荧光光度法并考察了影响二者包络作用的因素。在优化的条件下，线性范围为8.0×10^-8—1.0×10^-5mol／L，检出限2.5×10^-8mol／L。该方法用于渠水中微量溴敌隆的测定，回收率为92.2％-109.4％。

The supramolecular inclusion interaction between β-cyclodextrin（β-CD） and bromadiolone （BRD）, as an anticoagulant rodenticide was studied by spectrometry. The results showed that bromadiolone could bind with β-CD to fonn an inclusion complex with an association constant of 357.7 L/mol and a 1：1 stoichiometry. The related inclusion mechanism was proposed to explain the inclusion process. It was indicated that the hydrophobic group of bmmadiolone molecular, 4-hydmxycoumarin ring or Br substituted biphenyl, entered into the hydmphobic cavity of β-CD. At the same time, it was also observed the significant enhancement of relative fluorescence intensity of bromadiolone in the inclusion complex. Various factors affecting the inclusion pmcess were examined in detail. According to fluorescence enhancement phenomenon, a spectrofluorimetric method for determination of bmmadiolone in aqueous media was estab-lished with the linear range of 8.0×10^-8-1.0×10^-5mol/L and the detection limit of 2.5×10^-8mol/L. The proposed method was rapid, simple and direct. It was successfully applied to determining the trace amount of bmmadiolone in conduit water and the recoveries were in the range of 92.2% to 109.4%.