高效液相色谱化学发光法测定血清中左羟丙哌嗪

Determination of levodropropizine by reversed phase high performance liquid chromatography with chemiluminescence detection

在H2SO4介质中，KMnO4氧化左羟丙哌嗪产生化学发光，甲醛可增敏上述化学发光，在此基础上建立了反相高效液相色谱（RP-HPLC）分离，柱后化学发光检测左羟丙哌嗪的新方法，并成功应用于血清中左羟丙哌嗪的测定。在优化的实验条件下，该法测定左羟丙哌嗪的线性范围为1.0×10^-7—1.0×10^-5g／mL，检出限（3σ）为3×10^-8g／mL。对2.0×10^-6g／mL的左羟丙哌嗪进行11次平行测定，其相对标准偏差为2.3％。

A novel method was developed for the determination of levodroproizine by high performance liquid chroma-tography （HPLC） with chemiluminescence （CL） detection. The procedure was based on the chemiluminescence reaction of levodroproizine and potassium permanganate with formaldehyde as an enhancement reagent in a sulfuric acid medium. The chromatographic separation was performed on a nucleosil RP-C18 column （i.d. ： 250 mm×4.6 mm, particle size： 5μm, pore size： 100） with an isocratic mobile phase consisting of methanol and 0.1 mol/L NaH2PO4（volume ratio 45：55） at a flow rate of 1.0 mL/min. Under optimum conditions, the linear range for levodroproizine was 1.0×10^-7—1.0×10^-5 g/mL with the detection limit of 3×10^-8 g/mL（3σ）. The relative standard deviation （RSD） was 2.3% （n= 11） for 2.0×10^-6g/mL levodroproizine. Levodroproizine in the serum samples were analyzed by the proposed method with satisfactory results.