文冠果组织培养技术关键环节研究进展与展望

Research Advances and Future Prospect in the Key Link of Tissue Culture of Xanthoceras sorbifolia

文冠果(Xanthoceras sorbifolia Bunge.)属于组织培养繁殖困难的树种,问题的有效手段,逐渐引起人们的关注。作者从茎段培养、体细胞胚培养及愈伤组织培养三个方面对文冠果组织培养过程中外植体选择与处理、培养基的配制与激素处理及愈伤组织的诱导与继代培养等关键技术的研究现状和取得的成果进行了系统总结,介绍了目前报道的成功技术方案,分析了现有技术途径的效果和实用性,在此基础上明确了当前文冠果组培技术中存在的4项关键技术难题,并提出了今后文冠果组培技术研究中应重点加强的4个问题和方向。

Xanthoceras sorbifolia Bunge. is one of the tree species which is hard to propagate by tissue culture, an effective means to the question, gradually is caused for concern. This article systematically summarized the key technology in research and the acquired results from the stem segment culture, somatic embryo culture and callus tissue culture in the process of Xanthoceras sorbifolia tissue culture, such as explant selection and treatment, the preparation of medium and hormone treatment as well as callus induction and subculture etc., introduced the successful technical solutions reported currently, analyzed the effectiveness and practicality existing technical means, on the basis of this, clearly indicated the four key technical problems that existed in tissue culture of Xanthoceras sorbifolia, and proposed that should focus on strengthening the four questions and direction in the future research in the tissue cuhure of Xanthoceras sorbifolia.