温度对龙眼体胚发生早期POD活性及同工酶的影响

Effects of Different Temperatures on POD Activities and Isozymatic Patterns during Early Somatic Embryogenesis in Longan

以龙眼品种红核子LC2胚性细胞系诱导获得的体胚发生早期过程中不同发育阶段的同步化胚性培养物为材料,2个温度梯度(20℃、30℃)处理,以25℃为对照,初步研究了POD活性及同工酶在龙眼体胚发生早期的变化规律。结果表明:不同温度处理的胚性培养物,随着胚性细胞的分化,POD活性总体呈下降趋势,且无论高温(30℃)或低温(20℃)处理后龙眼体胚发生早期各阶段胚性培养物的POD活性均明显大于对照(25℃);温度处理对龙眼体胚发生早期POD同工酶谱影响也较大,表现为新谱带的出现。推测POD活性与同工酶谱的规律变化与龙眼体胚发生早期细胞分化以及维持体胚正常发育有密切关系。

The LC2 embryogenic cell line of the cuhivar Honghezi of longan was used as materials for obtaining the synchronized embryogenic cultures of early somatic embryogenesis at different developmental stages which were treated with different temperatures （20 ℃ and 30℃, compared with 25 ℃） for analyses of the peroxidase （POD） activities and isozymatic patterns during longan early somatic embryogenesis. The results showed that with the differentiation of embryogenie callus, all the POD activities of the embryogenic cultures treated with three different temperatures decreased, and the POD activitives of the embryogenic cultures treated with high （30℃ ） or relatively low （20 ℃ ） temperatures at the successive developmental stages during early somatic embryogenesis showed higher than the comparison （25 ℃ ）; the treatment of the temperature also had a great impact on the POD isozymatic patterns with the performance of the new bands for the emergence, which might be concluded that the changes of POD activities and isozymatie patterns in a law played a key role in the differentiation of early somatic embryogenesis, and keeping the normal development of somatic embryogenesis in longan.