海南肾茶的组织培养快速繁殖

Research about Rapid Propagation of Clerodendranthus spicatus (Thunb.) C.Y.Wu in Hainan through Tissue Culture

研究肾茶组织培养快繁的最佳材料、最佳激素配比、最佳培养基及炼苗移栽技术,以满足产业化生产肾茶对种苗的需求。以海南产肾茶幼嫩茎段的茎尖、茎节、茎间、叶片等为外植体,以MS为基本培养基,比较不同浓度和不同种类的生长激素对不定芽的萌发、愈伤组织增殖及丛生芽生根的影响。得出外植体消毒方法可采用70%的乙醇和0.1%的HgCl2溶液分时段来进行;最佳材料是茎尖;适宜的诱导培养基为MS+NAA0.1mg/L+6-BA1.0mg/L,继代增殖培养基为MS+NAA0.5mg/L+6-BA1.0mg/L或2,4-D0.5mg/L+6-BA1.0mg/L,生根培养基为1/2MS+CM10%+NAA0.1mg/L;成功建立了海南肾茶组织培养快繁技术体系,掌握了炼苗和移栽的相关技术。

Studies the best material and the best hormone matching and the best medium on tissue culture and practices seeding and transplant of Clerodendranthus spicatus, in Hainan. Satisfies the industrial production tea of Clerodendranthus spicatus. To seedling＇s demand. Using the stem tips and the stem nodes and the inter-nodes and the new leaves of Clerodendranthus spicatus. In Hainan as explants. Using MS as basic medium, the effects of different concentrations and different types of phytohormone on the germination of adventitious bud, proliferation of callus and rooting of tufty buds were compared. The explants disinfection method to be possible to use 70% ethyl alcohol and 0.1% HgCl2 solution minute time interval carries on; The best material is the stem point; The optimum medium for induction was MS＋ NAA 0.1 mg/L ＋6-BA 1.0 mg/L, the optimum medium for subculture proliferation was MS＋ NAA 0.5 mg/L＋6-BA 1.0 mg/L or 2,4-D 0.5 mg/L＋ 6-BA 1.0 mg/L and the optimum medium for rooting of stem segment was 1/2MS＋ CM 10%＋NAA0.1mg/L. The success has established the Clerodendranthus spicatus in Hainan tissue culture quick numerous technology systems, grasped has practiced the related technology which seeding and transplanted.