摘要
针对中国大豆灰斑病1号生理小种,以抗所有生理小种的品系东农40566为母本,以感所有生理小种的品种东农410为父本配制杂交组合,杂交得到F2代后连续自交3代得到F5代群体。该群体经人工接种灰斑病1号生理小种后,利用BSA法对500个SSR标记进行筛选,其中3个标记Satt565、SOYGPATR和Satt396在抗、感池间表现出稳定的多态性,并且在F2代个体中表现出抗性与多态性协同分离的趋势。3个标记与抗性基因的连锁顺序为Satt565—SOYGPATR—Hrcs1—Satt396,其与抗性基因的连锁距离分别为12.7、6.5、14.7cM。推测抗大豆灰斑病1号生理小种的基因可能位于C1连锁群上。
In aim to find the SSR markers linked to resistance gene against frogeye leaf spot in Chinese soybean, Bulked segregant analysis was applied to F5 populations derived from cross between the resistant line DongNong40566 and the susceptible line DongNong410, self-crossing for three generations after F2. These SSR markers including Satt565, SOYGPATR and Satt396 were identified from500 SSR markers, the linkage order was Satt565-SOYGPATR-Hrcsl-Satt396, and the distance to the resistance locus was 12.7, 6.5, 14.7 cM respectively. These three markers and the resistance locus were mapped to soybean linkage group C1.
出处
《中国农学通报》
CSCD
北大核心
2009年第9期43-46,共4页
Chinese Agricultural Science Bulletin
