无病毒百合组培种球快速繁殖体系的建立

Establishment of In vitro Rapid Propagation System on Virus-free Bulblets of Lilium spp.

以铁炮百合(Lilium Longiflorum)感病种球为材料,对热处理后的种球进行了培养基附加物和移栽基质的优化,建立了无病毒组培种球快速繁殖体系。结果表明:以MS为基本培养基,当附加植物激素NAA和KT时能促进子球再生,再生率达86%以上,在0.2mg/LNAA和0.4mg/LKT的培养基中平均再生子球数显著增多;附加5mg/L多效唑培养基能促进子球增大,3mg/L多效唑有利于子球再分化;活性炭对子球增大无明显影响,但适当浓度的活性炭能提高培养子球的品质;高浓度蔗糖能促进子球增大,株的但高增对,加子但,球在处再珍理分珠后化岩培有∶养土抑鳞为制片3影∶的1响作成。为活蛭基率石质和∶移土子栽、球珍组再珠培生岩球率以,显其及著成珍下活珠降率岩,和但︰生处土长理作状为2况0基天最质子佳时球。,再3均7生能℃率达热只到处能较理达高随4的着0%成处左活理右率天,但数和LSV检出率明显低于未处理的种球,脱毒率达95%以上。

Virus-free in vitro bulblet rapid propagation system was established using Lilium Longiflorum as experimental material. The results showed that the best medium for bulblets regeneration from bulb scales was MS＋KT 0.4 mg/L＋NAA0.2 mg/L. Optimum bulblet enlargement was observed in medium with Paclobutrazol 5mg/L, while Paclobutrazol in concentration 3 mg/L was propitious to bulblet formation. The quality of bulblets can be enhanced in the medium with appropriate concentration of AC. Medium with 90g/L sucrose can promote bulblet enlargement but inhibit bulblet formation. The best matrix for the highest surviving rate and best growth situation was pearlite/soil（3 ： 1）. About 50% bulb scales survived and regenerated after the treatment of high temperature 37℃ for 20 days, the effectiveness of eliminating virus with the method got to more than 95%.