HPLC法测定复方银翘氨敏胶囊中对乙酰氨基酚的含量

Determination of Paracetamol in Compound Yinqiao and Paracetamol Chlorphenamine Maleate Capsules by HPLC

目的:用HPLC法测定复方银翘氨敏胶囊中对乙酰氨基酚的含量。方法:采用C18柱(250mm×4.6mm,5μm),流动相为乙腈-水(10∶90),检测波长为249nm。结果:对乙酰氨基酚在2～50mg·mL-1范围内线性关系良好,r=1,平均回收率为100.95%(n=9),RSD为0.37%。结论:该方法简便、快速、准确,可以有效地控制药品质量。

Objective：To establish a method for determination of Paracetamol in Compound Yinqiao Paracetamol Chlorphenamine Maleate Capsules. Methods：The RP-HPLC carried out with Diamonsil C18 （4. 6 mm × 250 mm, 5μm） column and acetonitrile-water （ 10： 90） as the mobile phase, the UV detection wavelength was set at 249 nm. Result：Paracetamol showed a good linear relationship in the range of 2 - 50 mg·L^- 1 （ r = 1 ） . The average recovery （ n = 9 ） was 100. 95 % with RSD 0. 37 %. Conclusion： The proposed method was simple and convenient in operation. It is accurate and very suitable for the quality control.