摘要
15℃下,利用茎汁质量3.5%的纳米TiO2吸附茎菠萝蛋白酶,酶吸附率达98.2%,用0.4mol/L、pH6.0的柠檬酸缓冲液洗脱,酶回收率达79.3%,洗脱液先用截留值50ku的超滤膜超滤,流出液再用截留值10ku的超滤膜超滤,最后得截留液中茎菠萝蛋白酶的比活力2393U/mg蛋白,酶回收率为64.7%,纯化5.3倍,酶液浓缩10倍。
At the temperarure 15 ℃, 98.2% of stem bromelain activity is absorbed by 3.5% of na no-TiO2（by weight）. 79.3% of stem bromelain activity is eluted by 0.4 mol/L citrate buffer solution（pH6.0）. Ultrafiltration is applied to the elution with a membrane of 50 ku, and the outlet is collected and re-filtrated by a membrane of 10 ku. The specific activity of the remaining solution is 2393 U/mg protein, the activity recovery ratio is 64.7%, purification rate is 5.3. The solution is condensed 10 times in the meantime.
出处
《食品科技》
CAS
北大核心
2009年第4期167-170,共4页
Food Science and Technology
基金
科技部农业科技成果转化资金(2006C40038)
