摘要
采用圆二色谱(CD)方法对镧离子(La3+)与转移核糖核酸(tRNA)的相互作用进行了研究.结果显示La3+可使酵母tRNA的CD谱峰向短波方向偏移3nm,波峰位于254nm,峰值减少10%,说明结合在tRNA分子上的La3+引起tRNA分子构象的变化.La3+对氨酰化tRNA合成酶(aaRS)或tRNA-aaRS复合物的CD光谱基本上无影响,说明La3+对氨酰化反应活性的影响。
The interaction of Lanthanum(Ⅲ) ion(La^3+) with yeast tRNA was studied by circular dichroism(CD) spectrum.The results show that the peak position of yeast tRNA was slightly shifted towards short wavelength by 3 nm in the presence of 2 μmol/L La^3+,iefrom 257-254 nm;and the amplitude of positive peak at 254 nm was decreased by about 10%.The conformational change of yeast tRNA was caused by the binding of La^3+ to tRNA.La3+ did not change the CD spectra of either yeast aminoacyltRNA synthetase(aaRS) or tRNAaaRS complex,which suggests that the effects of La^3+ on the aminoacylation reaction activity may mainly be due to the conformational change of tRNA.
出处
《中山大学学报(自然科学版)》
CAS
CSCD
北大核心
1998年第1期5-8,共4页
Acta Scientiarum Naturalium Universitatis Sunyatseni
基金
国家攀登计划
国家教委博士点基金
马灿安教育基金
关键词
转移核糖核酸
镧离子
圆二色谱
酵母
transfer ribonucleic acid,lanthanum ion,circular dichroism spectrum,biological macromolecule