γ干扰素体外诱导大鼠脾脏树突状细胞IDO表达的实验研究

Study of γ-interferon-induced Expression of Indoleamine 2,3-Dioxygenase of Rat Spleen-derived Dendritic Cells in Vitro

目的探讨γ干扰素(IFN-γ)诱导大鼠脾脏树突状细胞(DC)的吲哚胺2,3-双加氧酶(IDO)的表达情况。方法大鼠脾脏分离培养DC,进行形态特征观察,流式细胞术检测DC的表型及确定其纯度。分别用不同浓度的IFN-γ诱导作用DC后,荧光定量RT-PCR测定IDO mRNA的相对表达水平。结果大鼠脾脏分离培养的DC纯度可达80%以上,培养10d的DC具有典型的树枝状突起,CD80、CD86的阳性表达分别达75%、90%以上。IDO mRNA的表达水平随IFN-γ的浓度的增大逐渐增加。结论IFN-γ在体外能诱导大鼠脾脏DC的IDO表达。

Objective To study the effect of γ-interferon（IFN-γ ） on the indoleamine 2,3-dioxygenase（IDO） expression in rat spleen-derived dendritic cells（DC ）. Methods DC were isolated and cultured from spleen in rat. The morphology of DC observed by microphotograph. The purity and phenotype were analyzed with flow cytometry. Separately with different concentrations of IFN-γ induced DC, relative expression of IDO mRNA were deteetec by fluorescence quantitative RT-PCR. Results The purity of cultured DCs above 80%.DCs cultured at 10 days with typical dendritic morphogy. The positive expression of CD80 and CD86 were more than 75% and 90%.Expressions of IDO mRNA with the concentration of IFN- γ increased gradually increase. Conclusion IFN- γ could induce the expression of IDO of rat spleen-derived DC in vitro.