利用花粉管通道转化谷子DNAj基因获得转基因小麦

Transformation of Wheat with DNAj Gene from Foxtail Millet via Pollen-tube Pathway

为提高小麦的抗旱能力,采用花粉管通道法将本实验室克隆的谷子抗逆相关基因DNAj转入小麦中。对授粉方式、柱头处理方式、质粒DNA浓度及DNA稀释试剂等因素对结实率的影响进行了对比,结果只有授粉方式对结实率的影响有显著差异,其他条件下结实率均未显示显著差异。对转化获得的703株T0小麦植株提取DNA进行分子检测,有1株小麦稳定扩增到了预期的1 260 bp的目的基因片段。虽然转化效率偏低(1.42‰),但说明利用花粉管通道法将谷子的抗逆相关基因DNAj导入小麦是可行的,为创造抗逆性改良的小麦新材料奠定了基础。

In order to obtain demonstrated to be related with drought-tolerant transgenic wheat drought and heat tolerant, was tried to pathway. The effects on seed setting under different pollination methods lines, DNAj gene from foxtail millet, which was introduce into 4 wheat cultivars via the pollen-tube , different treatments with stigmas, DNA concentra- tion and DNA diluents were studied and only the method of pollination sho^ed distinct influence on the seed setting rate. Genomic PCR of To generation plants revealed that the DNAj gene from foxtail millet was integrated into one wheat plant by repeated assay. This indicated it was feasible to introduce DNAj gene from foxtail millet into wheat via the pollen-tube pathway even the transgenic efficiency was only 1.42%e. Our work has established the foundation for creating drought-tol-erant wheat materials with foxtail millet genes.