柞蚕核型多角体病毒p11基因克隆及序列分析

Cloning and Sequence Analysis of Antheraea pernyi Nucleopolyhedrovirus(ApNPV)p11 Gene

为丰富柞蚕核型多角体病毒分子生物学基础，通过PCR扩增克隆了ApNPV p11基因并进行了序列的生物信息学分析。ApNPV p11基因编码102个氨基酸，预测分子量11．2kDa；氨基酸序列N端1～33位是信号肽序列，中部50～72位是跨膜区。PSI—BLAST搜索表明有20种核型多角体病毒编码蛋白与ApNPVP11蛋白有显著性匹配；比对分析发现P11蛋白氨基酸序列中部相对保守，两端变异较大。进化分析表明ApNPV属于NPV类群I且与EppoNPV、AgMNPV、CfDefNPV亲缘关系较近。

To enrich the molecular biology information of Antheraea pernyi nucleopolyhedrovirus（ApNPV）, the p11 gene was PCR amplified and cloned. Moreover, the sequence was subjected to bioinformatics analysis. ApNPV p11 gene encoded a 102 amino acid sequence with a predicted molecular weight of 11.2 kDa. The position 1 - 33 of the N terminal was the signal peptide sequence and the middle position（50- 72）was the transmembrane domain. PSI-BLAST search re- vealed that total 20 nucleopolyhedrovirus proteins significantly match with ApNPV Pll protein. Align analysis indicated that the middle parts was relatively conserved and the two ends were less conserved. Phylogenetic analysis classified ApN- PV as one group I NPV and closely related to EppoNPV, AgMNPV and CfDefNPV.