鸡猪源大肠杆菌CTX-M型ESBLs的分子检测

Molecular Detection of CTX-M Type ESBLs Genes among Escherichia coli Strains Isolated from Diseased Chickens and Pigs

为了解河南地区鸡猪源CTX-M型超广谱β-内酰胺酶(ESBLs)的流行情况,并进行基因型分析,通过表型确证试验检测产ESBLs大肠杆菌,分别用5组CTX-M型引物对其进行聚合酶链式反应扩增。表型确证试验检测结果显示,鸡、猪源大肠杆菌中产ESBLs菌株检出率分别为62.8%(59/94)和78.3%(36/46)。PCR扩增结果进一步显示,鸡源大肠杆菌中CTX-M型ESBLs阳性率为89.8%(53/59),其中CTX-M-2组型14株,CTX-M-9组型26株,CTX-M-2和CTX-M-9组型13株;猪源大肠杆菌CTX-M型ESBLs的阳性检出率为69.4%(25/36),其中,CTX-M-2组型7株,CTX-M-9组型17株,CTX-M-2和CTX-M-9组型1株。河南地区鸡、猪源大肠杆菌CTX-M型ESBLs的产生率较高,而以CTX-M-2组型和CTX-M-9组型为主。

In order to investigate the epidemiology of extended-spectrum β-1actamases-producing E. coli and to detect CTX-M type ESBLs in Henan province, the E. coli strains isolated from chickens and pigs were screened for the presence of ESBLs by phenotype confirmatory test, and theCTX-M type ESBLs were detected by PCR. The results indicated the positive rates of ESBLs among E. coli stains isolated from chickens and pigs were 62.8 % （59/94） and 78.3 % （36/46）, respectively. Further PCR amplification results revealed that the positive rate of the CTX-M type ESBLs in chickens was 89.8 % （53/59） . Of them, there were 14 isolates positive for CTX-M-2 group, 26 isolates for CTX-M-9 group, 13 isolates for both CTX-M-2 and CTX-M-9 group. While,the positive rate of the CTX-M type ESBLs in pigs was 69.44% （25/36）. Of them, there were seven isolates positive for CTX-M-2 group, 17 isolates for CTX-M-9 group, one isolate for both CTX-M-2 and CTX-M-9 group. The positive rate of CTX-M type ESBLs-producingEscheriehia coli strains is relatively high in Henan province, with the CTX-M-2 group and CTX-M-9 group dominant.