大鼠正畸牙移动过程中牙周组织NGF的表达变化

Change of nerve growth factor in periodontal tissue during orthodontic tooth movement

目的了解大鼠正畸牙移动过程中牙周组织内神经生长因子(nerve growth factor,NGF)的变化,探讨NGF在正畸牙移动过程中的作用。方法将85只雄性SD大鼠随机分为17组,其中空白对照组(0d组)、实验加力和对照不加力组各6h、12h、24h、3d、5d、7d、14d、21d组。选择左上第一磨牙作为实验牙,制备大鼠正畸牙移动不同时间牙周组织切片,进行免疫组织化学研究。结果正畸牙移动过程中,牙周组织内NGF表达发生改变。NGF表达量在正畸模型加载后迅速上调,实验组和对照组分别于5d、1d时表达水平达到最高,所有观察区域牙周膜内NGF表达显著增加,且实验组明显高于对照组。结论NGF在正畸牙移动过程中牙周组织改建的多个阶段起作用,参与了牙周膜早期的炎症反应及修复和晚期的改建。

Objective To observe the distribution and change of nerve growth factor（ NGF） in periodontal tissue and understand the role of NGF in orthodontic tooth movement.Methods Eighty-five male Sprague-Dawley rats were used. The animals were sacrificed after 0 d,6 h, 12 h,24 h,3 d,7 d,14 d and 21 days, respectively. Forces were applied on left maxinary first molar in rats,and the periodontal tissue was examined in different stages of tooth movement with immunohistochemical staining technique. Results NGF existed in normal periodontal tissue. The expression of it in periodontal tissue increased during orthodontic tooth movement, especially at the end of 5 days after force application. Conclusion NGF activity changed regularly and played an important role in remodeling of periodontal tissue during orthodontic tooth movement. NGF might participate in reconstruction of the periodontal tissue in early stage and regeneration of the periodontal tissue in late stage.