猪FSHβ和GDF9基因中Alu插入序列多态性

An Alu Insertion Polymorphism in the Porcine FSHβ and GDF9 Gene

使用聚合酶链式反应(Polymerase chain reaction,PCR)技术,对辽宁黑猪、长白猪、大约克猪、丹育猪和杜洛克猪的FSHβ亚基基因和GDF9基因的插入片段扩增后进行多态性和生物信息学分析,并对产仔数建立GLM模型,探讨2个基因插入序列Alu的多态性与猪产仔数的关系。研究表明:优势基因型BBDD纯合子比AACC型纯合子母猪平均每胎多产仔1.5头(P<0.01)。研究结果表明,FSHβ亚基基因和GDF9基因与控制猪产仔数的主效基因密切相关,这可能会作为猪产仔数性状的遗传标记,而其优势基因型BBDD可以进一步改良猪的产仔数性状。

The Alu insertion sequence of the porcine FSHβ and GDF9 genes were obtained using the PCR（Polymerase chain reaction） technique,from Liaoning Black,Landrace,Large White,Danyu Black and Duroc pigs,and the polymorphism was investigated,and the sequence was analyzed by bioinformatics in this study.The GLM model was constructed to study the relationship between litter size and the polymorphism of the Alu.The result showed that litter size of individuals with dominant genotype BBDD were 1.5 more than that with genotype AACC（P〈0.01）.Otherwise,the FSHβ and GDF9 genes were correlated deeply to the major gene influencing on the litter size,which may be considered as a genetic marker associating with pig litter size.The dominant genotype（BBDD） can improve the litter size traits.