两种组胺受体拮抗剂对内毒素诱导雏鸡肺损伤的影响

Effects of Two Histamine Receptor Antagonists on Lung Injury Induced by Endotoxin in Chicken

为探讨2种组胺受体拮抗剂在内毒素诱导肺损伤中的作用机理,作者设计了本试验。将120羽20日龄雏鸡随机分为5组,每组24羽。内毒素诱导肺损伤模型组每天早晚注射生理盐水0.5 mL.只-1,中午腹腔注射内毒素100μg.kg-1;扑尔敏组每天早晚肌注扑尔敏0.5 mL.只-1,中午腹腔注射内毒素100μg.kg-1;西咪替丁组每天早晚肌注西咪替丁0.5 mL.只-1,中午腹腔注射内毒素100μg.kg-1;扑西联合组每天早晚肌注西咪替丁、扑尔敏各0.3 mL.只-1,中午腹腔注射内毒素100μg.kg-1;空白组每天早中晚注射同等剂量生理盐水,连续15d。在第5、10、15天,各组随机抽7个样本,颈动脉放血处死,称取肺质量,采集肺脏以荧光法测组胺含量,制备病理切片观察病理变化并评分。结果发现:(1)第5天,扑西联合组肺组织中组胺含量显著低于扑尔敏组和西咪替丁组(P<0.05)。在第10天时,模型组肺组织中组胺含量显著高于扑西联合组和空白组(P<0.05);在第15天时,模型组肺组织中组胺含量显著(P<0.05)高于扑尔敏组和空白组,极显著(P<0.01)高于扑西联合组;(2)第10天时,扑尔敏组和空白组肺指数显著(P<0.05)或极显著(P<0.01)低于模型组,第15天时,扑尔敏组、扑西联合组和空白组肺指数显著(P<0.05)低于模型组;(3)第5、10、15天时,模型组肺病理评分分值与其余各试验组差异极显著(P<0.01),在第5天时,扑西联合组极显著低于扑尔敏组和西咪替丁组(P<0.01),在第10、15天时,扑西联合组显著低于(P<0.05)扑尔敏组,极显著低于其它各组(P<0.01)。结果表明,组胺通过H1、H2受体参与介导内毒素诱导雏鸡肺的损伤,但H1受体起主导作用。

The experiment was conducted to investigate the effects of two histamine receptor antagonists,chlorphenamine maleate and cimetidine,on acute lung injury induced by endotoxin in chickens.Methods： One hundred and twenty birds aged 20 d were randomly divided into five groups.（1） Endotoxin group（EG）： The normal saline was given twice on morning and afternoon,and endotoxin was given on noon;（2）Chlorphenamine maleate group（CM）： The chlorphenamine maleate was given twice on morning and afternoon,and endotoxin was given on noon;（3）Cimetidine group（CMT）： The cimetidine was given twice on morning and afternoon,and endotoxin was given on noon;（4）Chlorphenamine galeate and cimetidine group（CMC）： The chlorphenamine galeate and cimetidine was given twice on morning and afternoon,and endotoxin was given on noon;（5） Control group（CG）： The normal saline was given twice on morning and afternoon.Seven chickens were randomly taken from each group on the 5th,10th,15th day.The birds were weighed and killed.The lung was weighed,then it was fixed in neutral buffer formalin or stored in ultra cold freezer.The histamine was measured by OPT.Paraffin sections were made.Hematoxylin and eosin staining was used.The score criterion of pathology changes were measured.Results：（1） The contents of histamine in lung of CMC was lower than that of EG,CM and CMT.At the 5th day,CMC was significantly lower than that of CM and CMT（P〈0.05）.At the 10th day,EG was significantly（P〈0.05） higher than that of CMC and CG.At the 15th day,EG was significantly higher than that of CMC（P〈0.01）,CM and CG（P〈0.05）.（2） The index of lung in CM and CG was significantly less（P〈0.05） than that in EG at the 10th day,the index of lung in CM,CMC and CG was significantly less（P〈0.05）than that in EG at the 15th day.（3） The degree of lung injury of EG was significantly severer than other groups all the time（P〈0.01）,CMC was significantly lower than that of CM and CMT at the 5th day（P〈0.01）,but CMC was significantly lower than that of CM（P〈0.05） and CMT（P〈0.01） at the 10th,15th day.Those data indicated that histamine play roles in the endotoxin-induced lung injury through H1 and H2 receptors,but mainly through H1 receptor in chicken.