腈水合酶产生菌株的固定化载体选择

Carrier Selection for the Immobilization of Nitrile Hydratase Producing Strain

[目的]筛选适合腈水合酶产生菌株Rhodococcussp.HUST-3的最佳固定化载体。[方法]采用海藻酸钠、聚乙烯醇、聚丙烯酰胺、壳聚糖、琼脂进行腈水合酶产生菌株Rhodococcussp.HUST-3固定化载体研究,并进行海藻酸钠-聚乙烯醇、海藻酸钠-聚丙烯酰胺和海藻酸钠-壳聚糖复合固定化研究。[结果]结果表明,海藻酸钠-聚乙烯醇复合载体固定化腈水合酶产生菌株的相对酶活为93.1%,具有较好的机械强度,但固定化载体的反应批次较少,仅为7次;海藻酸钠-聚丙烯酰胺复合固定化载体具有较好的机械强度和耐磷酸盐程度,但是酶活相对较低,只有92.7%;海藻酸钠-壳聚糖复合固定化菌株的机械强度和耐磷酸盐性能都较高,相对酶活可达97.6%,能够进行12批次连续水合反应。[结论]海藻酸钠-壳聚糖为腈水合酶产生菌株的最适固定化载体。

[Objective]The research aimed to select the optimum immobilized carrier for nitrile hydratase producing strain.[Method]Nitrile hydratase producing strain Rhodococcus sp.HUST-3 was immobilized by sodium alginate,polyvinyl alcohol,polyacrylamide,chitosan and agar,and then combined.[Result]The results showed that the mechanical strength of sodium alginate-polyvinyl alcohol was high,but batch reaction was only 7 times.Sodium alginate-polyacrylamide had good performance in mechanical strength and phosphate-resistant,but relatively enzyme activity was only 92.7%.Alginate-chitosan microcapsles had high mechanical strength and phosphate-resistant performance,relatively enzyme activity reached 97.6%,and batch reaction could be conducted for 12 times.[Conclusion]Alginate-chitosan microcapsles was the optimum immobilized carrier for nitrile hydratase producing strain Rhodococcus sp.HUST-3.