高山杜鹃组培快繁技术研究

Study on Technology of Tissue Culture and Rapid Propagation for Rhododendron lapponicum

[目的]为高山杜鹃的快速繁殖提供技术指导。[方法]以高山杜鹃的侧芽为外植体,先将其在1/2MS培养基中预培养30 d,再对其进行芽增殖、芽伸长和生根培养,研究不同激素组合对其芽增殖、芽伸长和生根率的影响。[结果]当分裂素为6-BA时,每芽增殖数较少(最多为2.1个),当分裂素为KT时,芽增殖数随KT浓度的增加而增加,当KT为0.5 mg/L时,每芽增殖数最多(4.8个),且芽质量较好;培养基中KT/IAA为1/4时,芽最长(平均为5.1 cm),且芽较健壮;当培养基中加入IAA时,每株平均生根数较少(最多为1.2条),当加入NAA时,生根率随NAA浓度的增加而增加,当NAA为2.0 mg/L时,每株平均生根数最多(6.2条)。[结论]高山杜鹃组培苗增殖、芽伸长和生根的最佳培养基分别为MS+KT 0.5 mg/L+IAA 0.5 mg/L、MS+KT 0.25 mg/L+IAA 1.0 mg/L和1/2 MS+NAA2.0 mg/L。

[Objective] The aim was to provide the technical guidance for rapid propagation of Rhododendron lapponicum.[Method] With the lateral buds of R.lapponicum as the explants,they were pre-cultured on 1/2 MS medium for 30 d and then were cultured for bud propagation,bud elongation and rooting.The effects of different hormone combinations on bud propagation,bud elongation and rooting rate were investigated.[Result] When the mitogen was 6-BA,the propagation number per bud was less（the most was 2.1）.When the mitogen was KT,the propagation number per bud was increased with the increase of KT concn,and when KT.was 0.5 mg/L,the propagation number per bud was the most（4.8） with good quality.When the ratio of KT/IAA in medium was 1/4,the bud was the longest（being average of 5.1 cm） and strong.When IAA was added into medium,the rooting number per plant was less（the most was 1.2）.When NAA was added,the rooting rate was increased with the increase of NAA concn,and when NAA was 2.0 mg/L,the rooting number per plant was the most（6.2） [Conclusion] The optimal mediums for bud propagation,bud elongation and rooting of R.lapponicum were MS＋KT 0.5 mg/L＋IAA 0.5 mg/L,MS＋KT 0.25 mg/L＋IAA 1.0mg/L and 1/2 MS＋NAA 2.0 mg/L resp.