藻百年的组培快繁技术研究

Study on Tissue Culture and Rapid Propagation of Exacum tetragonum

[目的]研究藻百年(Exacum tetragonum)的离体培养和快速繁殖技术。[方法]利用藻百年的茎尖和茎段作为外植体进行组织培养和快速繁殖。[结果]结果表明,MS+6-BA2mg/L+NAA0.2 mg/L+蔗糖30 g/L+琼脂8 g/L为最适诱导培养基;MS+6-BA1 mg/L+NAA0.1 mg/L+蔗糖30 g/L+琼脂8 g/L为最适增殖培养基,1/2 MS+NAA0.3 mg/L+蔗糖30 g/L+琼脂8 g/L为最适生根培养基。[结论]该研究可为其他龙胆科植物的组织培养与快速繁殖提供方法参考。

[Objective]The research aimed to study the tissue culture and rapid propagation of Exacum tetragonum.[Method]The tissue culture and rapid propagation of E.tetragonum were carried out using stem apex and stem with buds as explants.[Result]The results showed that MS＋6-BA 2 mg/L＋ NAA 0.2 mg/L ＋ cane sugar 30 g/L＋ agar 8 g/L was optimum medium for induction.MS＋6-BA 1 mg/L＋NAA 0.1 mg/L ＋ cane sugar 30 g/L＋ agar 8 g/L was the optimum medium for reduplication.1/2 MS＋NAA 0.3 mg/L ＋ cane sugar 30 g/L＋ agar 8 g/L was optimum rooting medium.[Conclusion]This study could provide reference methods for the tissue culture and rapid propagation of other plants of Gentianaceae.