小麦导入磷酸烯醇式丙酮酸羧化酶(PEPCase)基因的初步研究

The Preliminary Research of Transferring PEPCase Gene into Wheat

[目的]将磷酸烯醇式丙酮酸羧化酶(PEPCase)基因导入普通小麦临优145。[方法]利用根癌农杆菌遗传转化系统,以普通小麦临优145为材料,将磷酸烯醇式丙酮酸羧化酶(PEPCase)基因,导入小麦胚性愈伤组织中,用含潮霉素(hyg)的筛选培养基连续筛选,并从分子水平上检测该基因。[结果]获得了hyg抗性植株,经GUS组织化学染色检测表明,抗性苗叶片被染成了深蓝色;PCR检测出目标条带。[结论]初步证明磷酸烯醇式丙酮酸羧化酶(PEPCase)基因已经导入受体材料中。

[Objective] The research aimed to transfer the PEPCase gene into wheat Linyou145.[Method] The PEPCase gene was transformed to embryonic callus in wheat Linyou145 by Agrobacterium tumefaciens-mediated transformation.Hygomycin Resistant（hyg） plants were obtained by screening in selective medium containing Hygomycin.The gene was detected on the lever of molecular.[Result] By means of PCR amplification and GUS histochemical analysis of Hyg-resistant plants showed that the target gene had been integrated into wheat accompany with hpt gene.Blue spots were observed in resistant leaves.[Conclusion] Its preliminary testified PEPCase gene had been transformed to wheat Linyou145.