碳纳米管与TritonX-100协同增敏Cu^(2+)与DBH-PF显色反应的机理研究

Study on the Synergic Effect of Carbon Nanotubes and TritonX-100 in the Chromogenic System of Cu^(2+)-Dibromohydroxy Phenylfluorone

研究了将碳纳米管(MWNTs)作为一种新型分析增效试剂与非离子表面活性剂曲拉通X-100(TritonX-100)的协同增敏作用机理,并应用于Cu2+与二溴羟基苯基荧光酮(DBH-PF)的络合显色体系中。结果表明,在MWNTs与TritonX-100复配的影响下,在pH=6.0~7.0磷酸盐缓冲溶液中DBH-PF与Cu2+形成红色络合物,其最大吸收红移至570 nm,吸收值提高了222.3%。Cu2+含量在1~10 0.04μg/mL范围内符合比耳定律,回归方程A=0.141C+0.028(C,0.04μg/mL),表观摩尔吸光系数ε=2.38×105L/(mol.cm),R=0.999 4。在最佳实验条件下,对中草药肉桂和黄连中的铜含量进行了测定,得到了满意的结果。

Here, the carbon nanotubes （MWNTs） were regarded as a novel kind of enhancing agent. A novel ehromogenic system was developed by the synergic effect of MWNTs and non-ionic surfaetants TritonX - 100, and the mechanism was discussed in this work. A red complex of Dibromohydroxy Phenylfluorone （DBH - PF） with Cu^2＋ was formed in the Phosphate buffer solution in a pH range of 6.0 - 7.0. Comparative experiments showed that, due to the synergic effect of MWNTs and TritonX - 100 the absorption of the red complexes increased by 222.3% and the λmax red shifted to 570 nm. The apparent molar absorptivity was 2.38 × 10s L/（mol · cm）. The linearity of the Cu^2＋ in mass concentration ranges from 1μg to 10 μg per 25 mL of solution with a correlation coefficient of 0. 999 4. This method has been successfully applied to the determination of trace Cu^2＋ in Chinese Herbal Medicine.