芪红胶囊通过免疫调节治疗病毒性心肌炎小鼠

The Immuno-Modulating Effect of Qi-Hong Capsule in the Treatment of Mouse Model of Viral Myocarditis Infected with Coxsackievirus B_3

目的观察芪红胶囊是否通过调节病毒性心肌炎小鼠的免疫系统发挥抗病毒作用。方法实验选用纯系雄性BALB/c小鼠,分组为:正常对照组、假处理对照组、利巴韦林对照组、芪红胶囊高、中、低剂量组,每组均为20只小鼠,腹腔注射10-3TCID50CVB3病毒造成病毒性心肌炎模型,4 h后灌胃给予芪红胶囊进行治疗,以利巴韦林作为阳性对照药物,于第3、6、9、12、15天随机选择每组中的4只动物进行实验。应用SABA18生化分析仪检测小鼠血清中LDH和CK-MB含量;通过芪红胶囊抑制VSV病毒在L929细胞上的毒力,测定芪红胶囊刺激脾细胞产生干扰素的效价;应用固定病毒稀释血清法测定血清中中和抗体浓度,最后取出小鼠心脏,制备心肌组织悬液,检测不同分组心脏组织中CVB病毒滴度。结果芪红胶囊能够显著降低血清中CK-MB和LDH含量(P<0.05),显示心脏受损程度减轻,并呈现量效关系;芪红胶囊具有诱生干扰素的能力,与病毒对照组间存在显著的统计学差异(P<0.05),利巴韦林组刺激干扰素产生的能力非常明显,优于芪红胶囊。此外,芪红胶囊具有刺激机体产生中和抗体的能力。对各组小鼠心肌组织悬液进行病毒滴度测定,发现病毒对照组小鼠的血清中,从第3天开始病毒滴度逐步上升,芪红胶囊药效组能够明显降低血清中的病毒滴度,在5个不同的时期均与病毒对照组存在显著的统计学差异(P<0.05),利巴韦林组也有明显的降低病毒滴度的作用(P<0.05)。结论芪红胶囊能够减少CVB病毒在心肌组织中的繁殖,其机制与芪红胶囊对小鼠免疫系统的调节有关。

Objective To investigate whether the traditional Chinese medicine Qi-Hong capsule has antiviral effect on the treatment of viral myocarditis in mice via modulating the immune system.Methods Male Balb/C mice were enrolled in this experiment and divided into 6 groups：normal control,sham control,mice treated with Ribavirin,mice treated with Qi-Hong capsule at three different concentrations.The mice were inoculated intraperitoneally with 10-3 TCID_50 CVB_3 virus suspension to simulate viral myoicarditis,and detected the immunological indexes on 3,6,9,12 and 15 d,including LDH,CK-MB,IFN and neutralizing antibodies.Lastly,the hearts were taken out and preparated into suspension to determine the titer of CVB_3.Results Qi-Hong capsules significantly decreased the concentration of CK-MB and LDH in the mouse serum（P〈0.05） and presented a dose-dependent response relationship,illuminating that Qi-Hong capsules could alleviate the cardiomyocyte lysis.The data of the IFN titer indicated that Qi-Hong capsules had strong ability to enhance the expression of IFN.In addition,Qi-Hong capsules could increase the content of neutralizaing antibodies in the mouse serum.The results of determination of the titer of CVB_3 in cardiomyocyte suspension showed that Qi-Hong capsules could significantly inhibit the replication of CVB_3 virus（P〈0.05） at different times.Conclusion Qi-Hong capsules may protect the mouse heart infected with CVB_3.The mechanism of this effect may be related to the modulation activity in immune system.